Identification of QTLs Controlling Radish Root Shape Using Multiple Populations

Root shape is an important characteristic that affects the commodity of radish (Raphanus sativus L.), which can be measured using ratio root length (RL) to diameter (RD). Although it known controlled by quantitative trait loci (QTLs), reliable QTLs for are still lacking. In present study, we used three F2 populations (1902, 1908 and 1909) derived from crossing five cultivars with highly divergent shapes perform QTL-seq. A total 1282 individuals were determine maximum diameter. High-depth resequencing six extreme pools parents was performed, QTL-seq detect controlling shape. We identified seven distributing on chromosomes (R1, R2, R4, R5 R7), among rs7.1 rs7.2 had overlap 1.02 Mb (13.79–14.81 Mb). addition, two QTLs, rs4.1 rs4.2, adjacent each other chromosome R4. conclusion, this study provides foundation fine mapping functional analysis breeding in radish.